Fig. 2.

Laccase activity of the wild-type Lac and chimeric Xyn-c-Lac toward different substrates. Enzymatic activity toward 2,6-DMP, ABTS, guaiacol, and VA was determined with different buffers in various pH conditions (pH value is given in parentheses under each bar pair). The oxidation rate was calculated based on the extinction coefficient of the oxidized product of each substrate. Each reaction was performed three times. Error bars represent SDs.