Table S1.
Data collection and refinement statistics
| Crystal form | SL12 RNP/9-nt substrate | SL12 RNP/13-nt substrate | SL13 RNP/11-nt substrate |
| Data collection | |||
| Space group | P41212 | P41212 | P1 |
| Cell dimensions | |||
| a, b, c (Å) | 242.676, 242.676, 146.898 | 241.781, 241.781, 145.002 | 97.916, 97.718, 128.793 |
| α, β, γ (°) | 90, 90, 90 | 90, 90, 90 | 108.98, 104.42, 89.86 |
| Wavelength (Å) | 0.9789 | 0.9789 | 0.97915 |
| Resolution range (Å) | 20–3.3 (3.36–3.3) | 20–3.6 (3.66–3.6) | 25–3.12 (3.17–3.12) |
| Unique reflections | 63,174 (2,486) | 49,349 (2,376) | 74,336 (3,707) |
| Redundancy | 7.2 (3.1) | 7.6 (4.0) | 2.3 (2.4) |
| <I>/<σ(I)> | 12.0 (1.2) | 12.4 (2.1) | 22.5 (4.1) |
| Completeness (%) | 96.5 (77.2) | 99.7 (97.4) | 97.3 (98.1) |
| Rmerge | 0.131 (0.729) | 0.165 (0.528) | 0.087 (0.514) |
| Structure refinement | |||
| Resolution range (Å) | 20–3.3 (3.36–3.3) | 20–3.6 (3.67–3.6) | 25–3.13 (3.21–3.13) |
| No. of reflections | 62,833 (2,130) | 49,034 (2,346) | 74,281 (5,061) |
| No. of atoms | 19,986 | 20,055 | 26,608 |
| Rwork | 0.250 (0.305) | 0.256 (0.305) | 0.178 (0.245) |
| Rfree | 0.303 (0.354) | 0.298 (0.340) | 0.234 (0.319) |
| Mean B (Å2) | 91.89 | 74.19 | 66.22 |
| rmsd bond length (Å) | 0.013 | 0.005 | 0.013 |
| rmsd bond angles (°) | 1.711 | 0.885 | 1.479 |
| Ramachandran plot | |||
| Favored (%) | 95.59 | 95.91 | 95.44 |
| Allowed (%) | 4.36 | 4.09 | 4.46 |
| Outliers (%) | 0.05 | 0 | 0.10 |
Values in parentheses are for the data in the highest-resolution shell.