Figure 3.

a–d. Tests standards (high, medium, and low) were tested on each IgA anti-β₂ GPI assay, and anti-human IgA conjugates were interchanged (blue from assay A and red from assay B).

There was a 4 times stronger reactivity of conjugate A when tested on assay B than conjugate B, suggesting that IgA detection in assay B is partially dependent on the IgA conjugate and calibration.

Standards A on assay A (a), standards B on assay B (b), standards A on assay B (c), standards B on assay B (d), and conjugate B did not elicit IgA anti-β₂ GPI antibody reactivity when tested on assay A (a–c) but only when tested on assay B (d)