Table 7. Regions identified in one S. Kentucky ST (ST152 or ST198) and absent or partially present in the other.
| ST | Region | Features | Size A | Flanking Loci in ST198 (CVM N51290 or ATCC 9263) B | Homologous Flanking Loci in ST152 (CVM29188) |
|---|---|---|---|---|---|
| 198 | 1 | histone acetyltransferase HPA2, hypothetical proteins | 4405 | AEX15_02835, AEX15_02865 | SeKA_A3272, SeKA_A3273 |
| 2 | putative superfamily I DNA helicases, putative restriction endonuclease, putative type II restriction enzyme methylase subunit, putative cytoplasmic protein, putative inner membrane protein | 24503 | SEEK9263_16827, SEEK9263_t17079 (tRNA-Leu) | SeKA_A4142 (tRNA-Leu), SeKA_A4161 | |
| 3 | sialic acid metabolism (transport) D | 9036 | AEX15_07510, AEX15_07550 (tRNA-Ser) | SeKA_A0529, SeKA_A0537 (tRNA-Ser) | |
| 4 | inositol catabolism | 23081 | AEX15_17525, AEX15_17625 | SeKA_A4089, SeKA_A4088 | |
| 5 | SPI-6 [Typhi colonization factor (tcf) operon] D | 8399 | AEX15_23130, AEX15_23170 | SeKA_A4630, SeKA_A4631 | |
| 6 | anaerobic dimethyl sulfoxide reductase chain A, B, C E | 13280 | AEX15_10355, AEX15_10415 C | SeKA_A1139, SeKA_A1140 C | |
| 7 | DNA-binding protein, phosphorylase, nucleotidyltransferas, exonuclease D | 9323 | AEX15_12395, AEX15_12455 (tRNA-Asn) | SeKA_A1557, SeKA_A1558 (tRNA-Asn) | |
| 8 | hypothetical protein (secretin domain/putative phage-related secreted protein) | 2459 | AEX15_09660, AEX15_09685 C | SeKA_A0993, SeKA_A1001 C | |
| 9 | carbon Starvation, D-gluconate and ketogluconates metabolism, Lactate utilization | 5674 | AEX15_10030, AEX15_10060 | SeKA_A1070, SeKA_A1073 | |
| 10 | SPI-13 with inserted iron reductase, FMN-dependent NADH-azoreductase, hypothetical proteins | 3314 | AEX15_04620 (tRNA-Phe), AEX15_04645 | SeKA_A2676 (tRNA-Phe), SeKA_A2689 | |
| 11 | hypothetical proteins, reverse transcriptase (adjacent to SPI-9) D | 4247 | AEX15_21645/AEX15_21650, AEX15_21675 C | SeKA_A2255 (tmRNA), SeKA_A2274 C | |
| 12 | glycine biosynthesis, glycine and serine utilization, serine-glyoxylate cycle, serine biosynthesis, glutamine, glutamate, aspartate and asparagine biosynthesis, threonine and homoserine biosynthesis | 3958 | AEX15_19945, AEX15_19970 | SeKA_A3752, SeKA_A3753 | |
| 13 | probable integrase remnant | 1050 | AEX15_01875, AEX15_01895 (tRNA-Ser) | SeKA_A0372, SeKA_A0399 (tRNA-Ser) | |
| 14 | type I restriction-modification system, putative inner membrane protein, hypothetical protein | 10325 | AEX15_20690, AEX15_20730 C | SeKA_A4188, SeKA_A4198 C | |
| 15 | hypothetical protein | 693 | AEX15_14050, AEX15_14060 | SeKA_A3461, SeKA_A3465 | |
| 16 | hypothetical proteins, phage tail protein, oxidoreductase, transglycosylase E | 25556 | AEX15_11375, AEX15_11530 | SeKA_A1348, SeKA_A1377 C | |
| ST | Region | Features | Size A | Flanking Loci in ST152 (CVM29188) | Homologous Flanking Loci in ST198 (CVM N51290 or ATCC 9263) |
| 152 | 1 | transcriptional regulator (LysR family) hypothetical protein, major facilitator superfamily MFS_1, 3-oxoacyl-[acyl-carrier-protein] reductase, transketolase, aldehyde-alcohol dehydrogenase 2 | 8040 | SeKA_A0275, SeKA_A0283 C | AEX15_01480, AEX15_01485 |
| 2 | Entner-Doudoroff pathway, glycolysis and gluconeogenesis, respiratory dehydrogenases 1, inositol catabolism, benzoate degradation D | 23239 | SeKA_A0372, SeKA_A0399 (tRNA-Ser) | AEX15_01875, AEX15_01895 (tRNA-Ser) | |
| 3 | hypothetical proteins D | 3951 | SeKA_A0529, SeKA_A0537 (tRNA-Ser) | AEX15_07510, AEX15_07550 (tRNA-Ser) | |
| 4 | hypothetical proteins, protein TolA | 4683 | SeKA_A0993, SeKA_A1001 C | AEX15_09685, AEX15_09660 C | |
| 5 | phage ORFs D | 33283 | SeKA_A1348, SeKA_A1377 C, F, G | AEX15_11530, AEX15_11375 C | |
| 6 | hypothetical proteins, protein YibA, protein RhsD, IS1-family insertion element D | 4847 | SeKA_A1701, SeKA_A1712 | AEX15_13120, AEX15_13125 | |
| 7 | hypothetical proteins, putative membrane protein, putative cytoplasmic proteins, transposases, HTH domain protein, integrase D | 11111 | SeKA_A2096, SeKA_A2114 C | AEX15_15380, AEX15_15440 C | |
| 8 | prophage integrase, hypothetical proteins, nuclease-related domain family protein, protein YpjI, DNA repair protein, toxin-antitoxin system (adjacent to SPI-9) D | 12402 | SeKA_A2255 (tmRNA), SeKA_A2274 C | AEX15_21645/AEX15_21650, AEX15_21675 C | |
| 9 | glycolysis and gluconeogenesis, mannitol Utilization | 5553 | SeKA_A2624, SeKA_A2631 | AEX15_04405, AEX15_04410 | |
| 10 | hypothetical protein, colicin-E7 immunity protein, bacteriophage integrase (fragments overlap with SPIs-8 and 13) D | 4943 | SeKA_A2676 (tRNA-Phe), SeKA_A2689 | AEX15_04620 (tRNA-Phe), AEX15_04645 | |
| 11 | hypothetical proteins, putative autotransporter | 5146 | SeKA_A3310, SeKA_A3314 | AEX15_02655, AEX15_02660 | |
| 12 | hypothetical proteins | 5073 | SeKA_A3672, SeKA_A3678 | AEX15_19610, AEX15_19615 | |
| 13 | DNA phosphorothioation, hypothetical proteins, integrase D | 17034 | SeKA_A4142 (tRNA-Leu), SeKA_A4161 C | SEEK9263_t17079 (tRNA-Leu), SEEK9263_16827 C | |
| 14 | type I restriction-modification system, putative inner membrane protein, hypothetical protein | 10240 | SeKA_A4188, SeKA_A4198 | AEX15_20690, AEX15_20730 | |
| 15 | oxidoreductase, inner membrane metabolite transport protein, sorbitol dehydrogenase, fructose-bisphosphate aldolase, kinase. | 9227 | SeKA_A0714, SeKA_A0725 C | AEX15_08380, AEX15_08375 C | |
| 16 | putative membrane transport protein, mandelate racemase/muconate lactonizing enzyme, putative transcriptional regulator | 3481 | SeKA_A3461, SeKA_A3465 | AEX15_14060, AEX15_14050 | |
| 17 | peroxisomal (S)-2-hydroxy-acid oxidase, putative glycolate oxidase | 1202 | SeKA_A1070, SeKA_A1073 | AEX15_10030, AEX15_10060 |
A = based on start/stop positions of proten encoding genes within region
B = flanking loci
C = encodes a string of homologous sequences but with unique flanking regions/may be unidirectional hits.
D = putative genomic island by as determined by IslandViewer3 [41].
E = part of this region predicted to be a genomic island by IslandViewer3 [41].
F = not detected in ARS-CC7487
G = truncated in CVM N45934