Table 2. Bactericidal activity of 50% NHS and 50% HIS decomplemented by heating at 56°C for 30 min against S. Enteritidis strains.
Bactericidal activity of 50% NHS1 | Bactericidal activity of 50% HIS2 | ||||||||
---|---|---|---|---|---|---|---|---|---|
Strain | CFU/ml3 | Survival rate of cells at T1 [%] | Survival rate of cells at T3 [%] | RP4 | CFU/ml | Survival rate of cells at T3 [%] | |||
T0 | T1 | T3 | T0 | T3 | |||||
27 | 2.36 x 106 ± 2.14 x 105 | 3.71 x 106 ± 8.52 x 105 | 9.07 x 106 ± 2.54 x 106 | 155.8 ± 23.2 | 379.9 ± 77.7 | R | 1.83 x 106 ± 2.32 x 105 | 1.67 x 108 ± 3.00 x 107 | 9092.2 ± 699.3 |
851 | 1.80 x 106 ± 1.05 x 105 | 3.28 x 106 ± 4.51 x 105 | 6.96 x 106 ± 6.91 x 105 | 183.2 ± 33.0 | 387.6 ± 48.8 | R | 2.74 x 106 ± 4.31 x 105 | 1.25 x 108 ± 4.26 x 107 | 4462.9 ± 851.2 |
670 | 2.40 x 106 ± 1.46 x 105 | 2.96 x 106 ± 3.30 x 105 | 4.84 x 106 ± 2.27 x 106 | 123.3 ± 7.3 | 202.3 ± 97 | R | 2.30 x 106 ± 1.36 x 105 | 3.77 x 107 ± 1.16 x 107 | 1624.9 ± 440.1 |
888 | 2.14 x 106 ± 1.75 x 105 | 2.07 x 106 ± 3.75 x 105 | 2.35 x 106 ± 3.27 x 105 | 96.2 ± 10.9 | 109.3 ± 6.6 | R | 3.06 x 106 ± 6.81 x 104 | 5.55 x 107 ± 3.80 x 106 | 1816.1 ± 99.8 |
1120 | 2.51 x 106 ± 2.80 x 105 | 2.43 x 106 ± 3.37 x 105 | 2.55 x 106 ± 3.14 x 105 | 96.5 ± 4.6 | 101.4± 2.1 | R | 1.60 x 106 ± 9.85 x 104 | 1.79 x 107 ± 3.67 x 106 | 1112.6 ± 174.2 |
351 | 3.15 x 106 ± 3.17 x 105 | 3.71 x 106 ± 2.76 x 105 | 6.77 x 106 ± 1.66 x 105 | 118.3 ± 10.0 | 216.3 ± 23.1 | R | 3.31 x 106 ± 2.88 x 105 | 3.36 x 107 ± 7.61 x 106 | 1016.7 ± 208.0 |
920 | 2.06 x 106 ± 7.51 x 104 | 2.53 x 106 ± 2.01 x 105 | 3.60 x 106 ± 5.11 x 105 | 123.0 ± 8.7 | 174.7 ± 22.1 | R | 1.92 x 106 ± 2.80 x 105 | 3.67 x 107 ±1.19 x 107 | 1885.3 ± 421.5 |
1227 | 1.54 x 106 ± 8.33 x 104 | 1.89 x 106 ± 1.54 x 105 | 2.57 x 106 ± 2.20 x 105 | 122.4 ± 5.5 | 166.5 ± 14.9 | R | 1.94 x 106 ± 5.16 x 105 | 4.98 x 107 ± 1.68 x 107 | 2568.2 ± 534.1 |
3686 | 2.48 x 106 ± 1.81 x 105 | 2.78 x 106 ± 3.50 x 105 | 3.79 x 106 ± 5.69 x 105 | 111. 9 ± 7.2 | 153.3 ± 20.6 | R | 4.02 x 106 ± 1.52 x 105 | 4.71 x 107 ± 3.92 x 106 | 1169.6 ± 82.0 |
4574 | 1.73 x 106 ± 1.61 x 105 | 2.02 x 106 ± 5.80 x 105 | 3.62 x 106 ± 1.18 x 106 | 115.5 ± 23.6 | 206.4± 51.2 | R | 2.78 x 106 ± 3.25 x 105 | 6.80 x 107 ± 2.06 x 107 | 2406.7 ± 469.3 |
1 Normal Human Serum
2 Human Inactivated Serum
3 Colony Forming Units/ml
4RP-Resistance phenotype: R – resistant strain, S – sensitive strain, I – intermediate strain.