Table 3. Bactericidal activity of 75% NHS and 75% HIS decomplemented by heating at 56°C for 30 min against S. Enteritidis strains.
Bactericidal activity of 75% NHS1 | Bactericidal activity of 75% HIS2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Strain | CFU/ml3 | Survival rateof cells at T1 [%] | Survival rateof cells at T3 [%] | RP4 | CFU/ml | Survival rate of cells at T3 [%] | ||||
T0 | T1 | T3 | T0 | T3 | ||||||
27 | 1.96 x 106 ± 1.59 x 105 | 2.26 x 106 ± 1.85 x 105 | 4.54 x 106 ± 3.36 x 105 | 115.2 ± 4.5 | 231.7 ± 14.8 | R | 2.04 x 106 ± 2.52 x 105 | 1.63 x 108 ± 2.99 x 107 | 8071.5 ± 1720.1 | |
851 | 1.47 x 106 ± 1.46 x 105 | 1.49 x 106 ± 2.25 x 105 | 3.90 x 106 ± 2.40 x 105 | 100.7 ± 9.5 | 265.4 ± 12.2 | R | 1.54 x 106 ± 2.14 x 105 | 2.23 x 107 ± 4.89 x 106 | 1446.8 ± 281.5 | |
670 | 4.93 x 106 ± 9.56 x 105 | 2.54 x 106 ± 5.20 x 104 | 2.31 x 106 ± 2.23 x 105 | 52.7 ± 9.5 | 47.5 ± 5.2 | S | 2.00 x 106 ± 1.51 x 105 | 3.64 x 107 ± 6.66 x 106 | 1809.9 ± 227.1 | |
888 | 5.59 x 106 ± 1.32 x 106 | 1.91 x 106 ± 1.31 x 105 | 5.35 x 105 ± 5.46 x 104 | 35.6 ± 9.1 | 9.9 ± 2.0 | S | 1.90 x 106 ± 4.01 x 105 | 2.14 x 107 ± 5.39 x 106 | 1122.8 ± 149.0 | |
1120 | 1.55 x 106 ± 1.10 x 105 | 2.69 x 105 ± 1.49 x 104 | 3.36 x 103 ± 8.50 x 102 | 17.4 ± 0.4 | 0.2 ± 0.1 | S | 2.48 x 106 ± 1.92 x 105 | 1.25 x 107 ± 4.50 x 106 | 497.3 ± 150.5 | |
351 | 1.51 x 106 ± 4.66 x 105 | 1.21 x 106 ± 3.53 x 105 | 1.18 x 106 ± 1.18 x 105 | 80.8 ± 2.6 | 81.6 ± 18,0 | I | 4.83 x 106 ± 5.80 x 105 | 3.43 x 107 ± 7.61 x 106 | 705.8 ± 84.7 | |
920 | 1.85 x 106 ± 2.59 x 105 | 1.99 x 106 ± 3.54 x 105 | 1.30 x 106 ± 2.78 x 105 | 107.2 ± 7.3 | 69.9 ± 5.8 | I | 1.87 x 106 ± 2.47 x 105 | 7.42 x 107 ±1.35 x 107 | 3981.8 ± 628.9 | |
1227 | 3.19 x 106 ± 2.75 x 105 | 1.65 x 106 ± 6.56 x 104 | 2.82 x 106 ± 3.29 x 105 | 52.1 ± 6.2 | 88.2 ± 5.2 | I | 5.99 x 106 ± 4.94 x 105 | 3.34 x 107 ± 2.49 x 107 | 536.6 ± 389.0 | |
3686 | 2.00 x 106 ± 5.06 x 105 | 1.87 x 106 ± 4.31 x 105 | 1.57 x 106 ± 3.72 x 105 | 94.2 ± 4.2 | 78.8 ± 1.2 | I | 2.01 x 106 ± 4.92 x 105 | 1.67 x 107 ± 4.61 x 106 | 850.3 ± 248.8 | |
4574 | 1.71 x 106 ± 3.93 x 105 | 1.64 x 106 ± 5.66 x 105 | 1.53 x 106 ± 2.84 x 106 | 94.3 ± 10.1 | 90.4 ± 3.8 | I | 2.06 x 106 ± 2.48 x 105 | 4.31 x 107 ± 4.21 x 106 | 2112.9 ± 341.9 |
1 Normal Human Serum
2 Human Inactivated Serum
3 Colony Forming Units/ml
4RP-Resistance phenotype: R – resistant strain, S – sensitive strain, I – intermediate strain.