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. 2016 Oct 3;11(10):e0163944. doi: 10.1371/journal.pone.0163944

Fig 3. Decreased frequency of intracellular TTR in CD14+ monocytes from FAP ATTR V30M patients.

Fig 3

CD14+ monocytes were isolated from PBMC of HD (n = 7) or FAP ATTR V30M patients (n = 6) using Ficoll-Paque and magnet bead-conjugated anti-human CD14 antibodies. (A, B) Separated CD14+ monocytes were stained with an anti-human TTR antibody. Five visual fields in each stained section were randomly chosen, and the number of TTR-positive CD14+ monocytes counted by three independent observers. The average count number per visual field is shown. The bar graph (A) shows the frequency of TTR+ cells in CD14+ monocytes. Photographs (B) show representative data for each group. Scale bars indicate 25 μm. (A, B) The proportion of repeated count TTR+ CD14+ monocytes were analyzed using the mixed model, with *p < 0.05 indicating a significant difference.