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. 2016 Oct 3;11(10):e0163295. doi: 10.1371/journal.pone.0163295

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© 2016 Zhou et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — Note: (A)The first step was to identify the 70^th plate pools had a positive clone. (B)The second step was to screen the 70^th plate and the 11^th columm had a positive clone. (C) The third step was to screen the the 11^th colummof the 70^th plate and BAC-10 was identified as a positive clone. (P) was the positive control containing pig genomic DNA. (N) was the negative control containing ddH₂O. M1: DL 2000 DNA ladder maker.