Table 5. The primers for screening PLE positive clones from Rongchang pig BAC library.
| ID | Primer(5’-3’) | annealing temperature(°C) | Products length(bps) |
|---|---|---|---|
| 1 | F-CAGGGCAGCCAGCCTCGCCGCCTGTTGTG
R-ATGGGAGGGTAGGAGGTGGTGTTCTTCAC |
62 | 208 |
| 2 | F-GCGATGCCCCAGAAGAGGAGGTCAGT
R-CAGCTCAGCATGCTTTATCTTGGGTG |
62 | 534 |
| 3 | F-GAACCCCAATGGGGAGGGGCTGCCCC
R-CAGCTCAGCATGCTTTATCTTGGGTG |
62 | 181 |
| 4 | F-TACCGCCTGGGCATCTGGGGATTCTTCAG
R-AGTTGCCCCGGCTGTGTTCATCCCCTGTG |
62 | 2327/3811 |
| 5 | F-CAGCAGGAGGGGAAAGTGTCTCTGTTCTG
R-AAGAGGTTCTTGGCCAAGGGAGACAACAC |
62 | 1540/3558 |
| 6 | F-TCAGGAAGGACATGAAGGCTGCAGCTAAG
R-GTTTTACACCCAGCAAGGACAGCAATTTG |
62 | 1869/1213 |
| 7 | F-ACGAGCTCTTGGACTTAACGCTGAAGATG
R-TCTCCATGCAAATCAAGAGTTAAAGGTTT |
62 | 2287/4056 |
| 8 | F-ATCTGTGACGGTGGCCCGTCAACACAGAG
R-CTCATACATGTAGGTGGGGGCTCCTGCAT |
62 | 3919/4758 |