Table 6. Reaction system of PCR.
| Ingredients | Sample volume (μL) |
|---|---|
| pig genome DNA templates (10ng/μL) | 0.5 |
| upstream primer (10μM) | 1.0 |
| downstream primer(10μM) | 1.0 |
| LA Taq enzyme | 1.0 |
| dNTP-MIX(10mM) | 8.0 |
| 10*Buffer(Mg2+) | 5.0 |
| ddH2O | 33.5 |
| Total | 50.0 |
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