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. 2016 Oct 3;11(10):e0162443. doi: 10.1371/journal.pone.0162443

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© 2016 Flowerika et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — A Maximum Parsimony based phylogenetic tree of the wheat and other monocots was generated by using MEGA 6 software. The sequences of O. sativa (PSY1, Q6KBZ6; PSY2, Q6ED35; PSY3, B6UV92), T. dicoccoides (PSY1, ACQ59135.1; PSY1, ACQ59141.1), T. urartu (PSY1, ACQ59127.1; PSY2, EMS46763.1; PSY3, EMS66563.1), T. monococcum (PSY, ACQ59129.1), T. turgidum (PSY1, ACO07290.1; PSY1, ABW80611.1; PSY2, A2T2L0), Ae. tauschii (PSY1, ACY68563.1; PSY2, EMT07172.1; PSY3 EMT04591.1), Ae. speltoides (PSY1, ACQ59147.1), H. vulgare (PSY1, M0YGH3; PSY2, M0WD98), B. distachyon (PSY1, I1GV47; PSY2, I1IG83), and Z. mays (PSY1, D1GIY3; PSY2, K7V3J4; PSY3, B0KYU8) were considered. The squalene synthase sequence of Ae. tauschii (EMT03383.1) was used as outlier. The sequences were aligned using MUSCLE and the phylogenetic tree was created by Neighbor-Joining method with 1000 bootstrap replicates using MEGA 6 software. The numbers shown at each node represent bootstrap values.