Table 1. Primer and probe sequences used for quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
Gene Symbol | NM_Number | Probe Name | Probe Sequence | Forward Name | Forward Sequence | Reverse Name | Reverse Sequence |
---|---|---|---|---|---|---|---|
RACGAP1 | NM_013277 | MP502 | ACTGAGAATCTCCACCCGGCGCA | MP502_For | TCGCCAACTGGATAAATTGGA | MP502_Rev | GAATGTGCGGAATCTGTTTGAG |
TOP2A | NM_001067 | MP495 | CAGATCAGGACCAAGATGGTTCCCACAT | MP495_For | CATTGAAGACGCTTCGTTATGG | MP495_Rev | CCAGTTGTGATGGATAAAATTAATCAG |
Ki67 | NM_002417 | MP552 | ACGGTCCCCACTTTCCCCTGAGC | MP552_For | CGAGACGCCTGGTTACTATCAA | MP552_Rev | GGATACGGATGTCACATTCAATACC |
CALM2 | NM_001743 | MP501 | TCGCGTCTCGGAAACCGGTAGC | MP501_For | GAGCGAGCTGAGTGGTTGTG | MP501_Rev | AGTCAGTTGGTCAGCCATGCT |