IIIL-1 is present (i.e., section 91 is inverted). A. Base of S. brevipar ‘C’ (Site 12). Limits of polymorphic inversions IIIL-58 of S. yuphae ‘B’ and IIIL-70, IIIL-73, IIIL-75, and IIIL-78 of the S. doipuiense complex are indicated by brackets. C = centromere; hb = location of heteroband of S. rufibasis ‘B’; hc and in = locations of heterochromatic block and of 2 fine band inserts, respectively, of S. doipuiense ‘A’. B. Distal portion of Simulium doipuiense ‘E’ (Site 15), showing the probable X-linked IIIL-68,69 sequence on top of IIIL-11,13, plus homozygous mild expression of heteroband 100hb1. The Simulium subgeneric standard sequence [19] can be obtained by alphabetically ordering the fragments indicated by the letters a–p; cs = cup and saucer marker.