IIIL-1 is present (i.e., section 91 is inverted). The sequence of the Simulium subgeneric standard [19] can be obtained by alphabetically ordering the fragments indicated by small letters; cs = cup and saucer marker. A. Simulium rufibasis ‘B’ (male larva, Site 12), showing the IIIL-8,11,13 sequence; subterminal inversion IIIL-61 is expressed heterozygously, and breakpoints of polymorphic inversions IIIL-60 and IIIL-64 are indicated by brackets; hb = location of heteroband. B. Simulium doipuiense ‘F’ (female larva, Site 15), showing the IIIL-11,13,87,88,89 sequence; the second breakpoint for IIIL-89 follows the inversion of IIIL-87, creating the d/j junction. Tentative breakpoints and section numbers are indicated with a bracket and asterisk (*) above them.