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. 2016 Oct 3;11(10):e0163437. doi: 10.1371/journal.pone.0163437

Fig 2. Optimizing SLB formation to reduce nonspecific binding.

Fig 2

a) SLBs were formed over 30 min using 1% aGM1 receptor-less lipid vesicles at varying concentrations (white text). X31 nonspecifically bound and spontaneously fused to SLBs (as noted by the radial release and diffusion of R18 into the supported bilayer) formed with low vesicle concentrations. b) The SLB formation time (white text) was varied while maintaining a constant 1 mg/mL vesicle concentration. Nonspecific binding was reduced greatly when SLBs are formed over at least 150 min. All images in panel a and b, were taken 30 min after loading the X31 virus.