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. 2016 Oct 3;11(10):e0163437. doi: 10.1371/journal.pone.0163437

Fig 3. STAWASP algorithm.

Fig 3

a) A local cluster of pixels (gray pixels) must either increase or decrease in intensity between consecutive frames to be classified as synced pixels. The pixel cluster size must be larger than a pixel and smaller than the particle of interest. Here, at least 80% of the gray pixels must be synced. b) The steps to determine synced pixels are portrayed here for a particle appearing at frame 6. Since synced pixels are determined using two adjacent frames, synced pixels are marked on both of those frames. Note that false synced pixels can be generated by random noise fluctuations, but these do not necessarily cause a false particle to appear. c) The intensity trace for the center pixel of the particle images part B is shown when using STAWASP or the regular 10-frame temporal averaging scheme. With STAWASP, temporal averaging is performed in a segmented fashion such that temporally adjacent, synced pixels are not averaged together.