| rabbit anti-FMRP |
1 in 2000 |
1 in 250 |
ab17722 |
Abcam, Cambridge, UK |
| mouse anti-OCT4 |
|
1 in 700 |
sc-5279 |
Santa Cruz Biotechnology, SantaCruz, USA |
| rabbit anti-LIN28 |
|
1 in 10,000 |
ab46020 |
Abcam Cambridge, UK |
| rabbit anti-SYCP3 |
|
1 in 50,000 |
ab150292 |
Abcam Cambridge, UK |
| rabbit anti-DCP1a |
|
1 in 6,000 |
ab47811 |
Abcam Cambridge, UK |
| rabbit anti-DDX6 |
|
1 in 8,000 |
A300-461A |
Cambridge Bioscience, Cambridge, UK |
| mouse anti-G3BP |
|
1 in 16,000 |
611126 |
BD Biosciences, Erembodegem, Belgium |
| mouse anti-GW182 |
1 in 500 |
sc-56314 |
Santa Cruz Biotechnology, Santa Cruz, USA |
| rabbit anti-PABP1 |
|
1 in 6,000 |
N/A |
from Prof. Nicola Gray |
| Secondary Antibodies |
|
|
|
|
| biotinylated goat anti-rabbit |
1 in 500 |
1 in 500 |
E0432 |
Dako, Cambridge, UK |
| biotinylated goat anti-mouse |
|
1 in 500 |
BA-9200 |
Vector, Peterborough, UK |
| Peroxidase-conjugated goat anti rabbit |
|
1 in 500 |
P0448 |
Dako, Cambridge, UK |
| Peroxidase-conjugated goat anti-mouse |
|
1 in 500 |
P0447 |
Dako, Cambridge, UK |
| Streptavidin-conjugated HRP |
1 in 1000 |
|
SA-5004 |
Vector, Peterborough, UK |
| Visualisation Dyes |
|
|
|
|
| streptavidin-conjugated Alexa Fluor 488 |
|
1 in 500 |
S-11223 |
Life Technologies Ltd, Paisley, UK |
| Cy3-conjugated Tyramide |
|
1 in 50 |
NEL744B001KT |
Perkin Elmer, Cambridge, UK |
| Cy5-conjugated Tyramide |
|
1 in 50 |
NEL745B001KT |
Perkin Elmer, Cambridge, UK |
| Liquid DAB + Substrate Chromogen System |
N/A |
|
K3468 |
Dako, Cambridge, UK |
| DAPI |
|
1 in 1000 |
D9542 |
Sigma-Aldrich, Dorset, UK |
