Table 1. Growth rate of C. neoformans wt and mutants.
| Aminoacid group | Nitrogen source | aap2Δ | aap4Δ | aap5Δ | aap4Δ/ aap5Δ | mup1Δ | mup3Δ | mup1Δ/ mup3Δ |
|---|---|---|---|---|---|---|---|---|
| Aliphatic | Alanine | - | - | - | - | - | - | - |
| Glycine | NS | 58% (*) | 60% (*) | NS | NS | NS | NS | |
| Isoleucine | 28% (***) | 27% (***) | 36% (***) | 22% (***) | NS | NS | NS | |
| Leucine | NS | NS | NS | 6% (***) | NS | NS | NS | |
| Valine | NS | 57% (**) | NS | 51% (**) | NS | NS | 43% (**) | |
| Hydroxylated | Serine | NS | 44% (**) | NS | 10% (***) | NS | NS | 58% (*) |
| Threonine | - | - | - | - | - | - | - | |
| Sulphur | Cysteine | - | - | - | - | - | - | - |
| Methionine | NS | 11% (**) | NS | NS | NS | NS | 30% (*) | |
| Acidic/Amide | Asparagine | NS | NS | NS | 51% (**) | NS | NS | NS |
| Aspartic acid | NS | 13% (***) | NS | 7% (***) | NS | NS | NS | |
| Glutamic acid | NS | NS | NS | 4% (***) | NS | NS | NS | |
| Glutamine | NS | NS | NS | 46% (**) | NS | NS | NS | |
| Basic5 | Arginine | 14% (***) | NS | NS | 20% (***) | NS | NS | NS |
| Histidine | - | - | - | - | - | - | - | |
| Lysine | 40% (***) | 36% (***) | NS | 11% (***) | NS | NS | NS | |
| Aromatic | Phenylalanine | NS | 49% (***) | NS | 42% (***) | NS | NS | NS |
| Tryptophan | NS | NS | NS | 58% (**) | 69% (*) | 34% (***) | 40% (***) | |
| Tyrosine | - | - | - | - | - | - | - | |
| Imino | Proline | NS | NS | 59% (**) | 32% (***) | 83% (*) | NS | 81% (**) |
Growth percentage of each permease mutant (single and double mutant) relative to that of the wild type (H99) on single amino acid as the sole nitrogen source at 30°C. Non-significant differences and no growth are denoted as “NS” and “-”, respectively; p value < 0.05 (*), 0.01 (**), 0.0001 (***).