Table 2. Growth rate of C. neoformans wt and mutants.
| Aminoacid group | Nitrogen source | aap2Δ | aap4Δ | aap5Δ | aap4Δ/ aap5Δ | mup1Δ | mup3Δ | mup1Δ/ mup3Δ |
|---|---|---|---|---|---|---|---|---|
| Aliphatic | Alanine | - | - | - | - | - | - | - |
| Glycine | 59% (***) | NS | NS | 0% (***) | 83% (*) | 75% (**) | 83% (*) | |
| Isoleucine | NS | NS | NS | 20% (***) | NS | NS | 55% (**) | |
| Leucine | NS | NS | NS | 6% (***) | NS | NS | NS | |
| Valine | NS | NS | 9% (***) | 4% (***) | 28% (***) | 8% (**) | 17% (***) | |
| Hydroxylated | Serine | NS | NS | NS | 12% (***) | NS | NS | NS |
| Threonine | - | - | - | - | - | - | - | |
| Sulphur | Cysteine | - | - | - | - | - | - | - |
| Methionine | 64% (**) | NS | 44% (***) | 4% (***) | 44% (***) | 20% (***) | 10% (***) | |
| Acidic/amide | Asparagine | 61% (***) | NS | NS | 0.4% (***) | 96% (*) | 81% (**) | 72% (***) |
| Aspartic acid | 30% (***) | 70% (**) | 59% (***) | 1% (***) | 72% (**) | 74% (**) | 60% (***) | |
| Glutamic acid | 58% (**) | 73% (**) | NS | 0.7% (***) | NS | NS | NS | |
| Glutamine | 77% (*) | NS | NS | 0% (***) | NS | NS | 79% (*) | |
| Basic | Arginine | 19% (***) | 72% (**) | NS | 3% (***) | NS | 75% (**) | 61% (***) |
| Histidine | - | - | - | - | - | - | - | |
| Lysine | 72% (**) | NS | NS | 4% (***) | NS | 81% (*) | NS | |
| Aromatic | Phenylalanine | 48% (***) | NS | 73% (**) | 0% (***) | NS | 78% (*) | 79% (*) |
| Tryptophan | 67% (***) | NS | NS | 0% (***) | NS | 82% (**) | 85% (**) | |
| Tyrosine | - | - | - | - | - | - | - | |
| Imino | Proline | NS | NS | 76% (**) | 58% (***) | 80% (*) | NS | 73% (**) |
Growth percentage of each permease mutant (single and double mutant) relative to that of the wild type (H99) on single amino acid as the sole nitrogen source at 37°C. Non-significant differences and no growth are denoted as “NS” and “-”, respectively; p value < 0.05 (*), 0.01 (**) and 0.001 (***).