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. 2016 Sep 15;143(18):3382–3393. doi: 10.1242/dev.136234

Fig. 5.

Fig. 5.

Hyper-accumulation of superoxide is required for callus formation in msl2 msl3 and msl2 msl3 p5cs1 seedlings. (A) NBT-stained seedlings provided with TEMPOL or NaCl at 0, 3 or 7 DAG. Scale bars: 1 mm. (B) Callus production in mutant seedlings when grown on MS, MS with 1 mM TEMPOL or MS with 82 mM NaCl. (C) Confocal micrographs of non-green plastids in the first true leaf of msl2 msl3 mutants harboring the RecA-dsRED plastid marker grown on the indicated medium. Scale bar: 10 µm. (D) Apical callus production in T2 lines segregating transgenes that overexpress CSD2 or mCSD2. The mean of four biological replicates (n≥35 seedlings each replicate) is presented. Error bars indicate s.d. Statistical grouping was performed by ANOVA followed by Tukey's HSD test, P<0.01. (E) Percentage of seedlings from D stained with NBT in three phenotypic categories (n≥20 seedlings). (F) Images of CSD2 overexpression T2 lines. White arrows indicate deformed leaves. Scale bars: 5 mm.