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. 2016 Sep 15;143(18):3328–3339. doi: 10.1242/dev.136465

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© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Expression pattern of RBOH genes during LR development and RBOH-mediated effect on LR emergence phenotype. (A) Promoter activities of RBOH genes during LRP development. Seven-day-old seedlings of each pRBOH:nlsGFP:GUS line, as indicated, were GUS stained. Scale bars: 50 μm. (B) Effect of the superoxide dismutases blocker diethyldithiocarbamate (DDC, 1 mM), and the RBOH inhibitor diphenyleneiodonium chloride (DPI, 1 μM) on LR emergence phenotype, starting from stage I to an emerged LR (E on x-axis). Data points represent mean±c.i. (in two biological replicates, n=20). (C,D) LR emergence phenotype in wild type and RBOH single and higher order mutants, as indicated. Data points represent mean±c.i. (in two biological replicates, n=20). (E) A synchronization of LRP initiation (20 h) and emergence (44 h) is achieved by gravistimulation and occurs at the bending site.