Fig. 6.
Cohesive forward movement of migrating CVM cell clusters is affected in embryos from gcl and wun wun2 maternal mutants. Temporal color-coded projections of CVM cell migration in wild-type (A,C), gclΔ/Df (B,D) and wun wun2 (E) embryos expressing the HC3 reporter. Each projection is compiled from 80 movie stills of time points taken every 3 min over a 4-h span (A′,B′), with each still assigned a unique color code corresponding to a specific time point. (A,A′,C) CVM migration in wild-type embryos proceeds in a cohesive yet dynamic fashion. Although some cells occasionally deviate towards the midline, these eventually rejoin and resume forward movement as part of a collective cluster. A′ and C are embryos 2 and 1, respectively, in Movie 3. By contrast, embryos from gcl maternal mutants migrate in a relatively undeviating and compact fashion (B,B′,D). B′ and D are embryos 2 and 3, respectively, in Movie 4. In wun wun2 mutants, some CVM cells wander towards the midline but fail to rejoin either of the two migrating clusters (E).