Extra longitudinal muscles are present in the midguts of larvae derived from gcl maternal mutant mothers. (A,B) Phalloidin staining of the proventriculus of third instar larval midguts to mark longitudinal and circular muscles. Longitudinal muscle runs left to right in panels. Dashed box indicates regions analyzed in C. (C) Quantification of the number of longitudinal muscles per half-side in the anterior midgut of the larvae. Red lines on the plot indicate the median value for each data set, which are significantly different (P=0.0048, Mann–Whitney–Wilcoxon test). yw females, n=25; gclΔ/Df maternal mutants, n=29.