Fig. 3.

Reducing Pros rescues the loss of INPs resulting from Pnt knockdown. (A-D″) Type II NB lineages are labeled with mCD8-GFP driven by pntP1-GAL4. Arrows, type II NBs; arrowheads, INPs. (A-B″) Wild-type (A-A″) or pros¹⁷ heterozygous (B-B″) brains. Each type II NB lineage has multiple mature INPs. (C-C″) INPs are largely eliminated in Pnt knockdown type II NB lineages. Note that most type II NBs become Ase⁺ (green arrows). (D-D″) INPs are produced in a subset of type II NB lineages and Ase remains suppressed in most type II NBs (arrows) when Pnt is knocked down in pros¹⁷/+ heterozygotes. (E-G) Quantification of the percentage of lineages with INPs (E), the number of INPs (F) and the percentage of lineages with Ase⁻ NBs (G). **P<0.01. Scale bars: 20 μm.