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Erm is largely abolished in Pnt knockdown type II NB lineages. (A-A″) Erm is expressed in Ase− and Ase+ imINPs (open arrows) in a wild-type type II NB lineage labeled by GFP. Arrows, type II NBs. (B-C″) Erm is not detected in Ase− imINPs (open arrows) or Ase+ progeny (open arrowheads) when Pnt is knocked down. (D) Quantification of Erm staining intensity. **P<0.01. Scale bars: 20 μm.
