Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Sep 1;129(17):3251–3261. doi: 10.1242/jcs.193367

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 2. — 4D observation of cisternal maturation. Wild-type cells expressing (A) Mnn9–mCherry (cis, magenta) and Gnt1–GFP (medial, green), (B) Mnn9–mCherry (cis, magenta) and Sys1–GFP (trans, green) and (C) mRFP–Sed5 (cis, magenta) and Sec7–GFP (trans, green) were grown to a mid-logarithmic phase in synthetic medium at 30°C and observed by SCLIM. Upper left panels show representative 3D images. Dashed lines indicate the edge of the cells. Upper right montages show 3D time-lapse images (4D) of the indicated squares. Lower left panels show the relative fluorescence intensities of green and red channels in the indicated cisterna. Lower right panels show magnified images of cells at selected time points. Scale bars: 1 µm. Representative images from at least 26 independent cisternae are shown.