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. 2016 Sep 1;129(17):3251–3261. doi: 10.1242/jcs.193367

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© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — Depletion of COPI protein inhibits cis to medial cisternal maturation. (A) Wild-type (WT), (B) Ret1-aid and (C) Sec21-aid cells expressing Mnn9–mCherry (cis, magenta) and Gnt1–GFP (medial, green) were grown to a mid-logarithmic phase in synthetic medium with or without 1 mM NAA at 30°C and observed by SCLIM. Representative 3D images of cells with (lower panels) or without (upper) NAA are shown. Dashed lines indicate the edge of cells. Right montages show 3D time-lapse images of the indicated areas. (D) Wild-type, Ret1-aid and Sec21-aid cells with NAA expressing Mnn9–mCherry (cis, magenta) and Gnt1–GFP (medial, green) were observed. Left panels show representative 3D images of cells. Dashed lines indicate the edge of cells. Right montages show 3D time-lapse images of the indicated areas. (E) Magnified images of selected time points in D are shown. Scale bars: 1 µm. (F) Bar graph shows the rate of maturation of cisternae from cis to medial. Error bars represent s.d. from at least five independent cells.