Fig. 7.

The number of growing microtubules increases in severed dendrites in a fidgetin-dependent manner. Movies of EB1–GFP dynamics were acquired in ddaC neurons expressing control (γTub37C RNAi), fidgetin or spastin RNA hairpins. EB1–GFP dots were counted in the cut-off part of dendrite arbor in injured animals or the whole dendrite arbor in uninjured ones, and the length of in-focus dendrites was measured. Total comet number was divided by length to give the average number of comets in a 10-μm stretch. (A) Comet number per 10-μm length is shown in uninjured or severed dendrites 1 h after laser surgery. ***P<0.001; n.s., not significant (unpaired t-test). (B) Example kymographs of EB1–GFP movement are shown.