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. 2016 Sep 1;129(17):3262–3273. doi: 10.1242/jcs.171124

Fig. 5.

Fig. 5.

K8/K18 IF modulation of PKCδ translocation to the surface membrane versus internalization. (A) Immunofluorescence confocal imaging of PKCδ–EGFP in transfected WT and K8-null hepatocytes treated with PMA (100 nM) and fixed at 1, 5 and 15 min post treatment. (B) Percentage assessments of WT or K8-null hepatocytes exhibiting a PKCδ–EGFP translocation at the surface membrane as a function of time after treatment with PMA (100 nM), showing a rapid translocation to the surface in both cell types, with even faster kinetics in hepatocytes lacking K8/K18 IFs (n>70). (C) Percentage assessments of WT or K8-null hepatocytes exhibiting a PKCα–EGFP translocation at the surface membrane as a function of time after a PMA treatment (100 nM), showing no difference in both cell types (n>60). (D) Lateral (XY) and axial (XZ) immunofluorescence confocal imaging of PKCδ–EGFP in transfected WT and K8-null hepatocytes treated with PMA (100 nM) and fixed at different time points, revealing less PKCδ–EGFP internalization in hepatocytes lacking K8/K18 IFs (CTRL: untreated control). Quantitative results are mean±s.e.m.