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. 2016 Sep 29;9:5931–5941. doi: 10.2147/OTT.S106288

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© 2016 Markowitz et al. This work is published and licensed by Dove Medical Press Limited

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Clinical workflow demonstrating inputs and research products for measuring microRNA (miRNA) in patients.

Notes: In this study, peripheral phlebotomy techniques were optimized such that it can be performed routinely without visible evidence of hemolysis. This permits the efficient separation of plasma followed by RNA extraction and miRNA measurement without contamination of miRNAs from lysed cells. The miRNA data obtained from RNA isolated from heparin phlebotomy tubes were subjected to complementary biostatistical and rank-based analytical approaches to identify miRNAs from patient plasma samples.