Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The BioTAP affinity tag, which combines both Protein A and a biotinylation acceptor sequence, was cloned into the N-terminus of the three different cDNAs. Tissue culture cells expressing the tagged baits were crosslinked with formaldehyde and sonicated prior to affinity pulldowns and on-bead derivatization.
