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. Author manuscript; available in PMC: 2016 Oct 3.
Published in final edited form as: FEBS Lett. 2007 Dec 27;582(2):291–298. doi: 10.1016/j.febslet.2007.12.021

Fig. 1.

Fig. 1

Rac/Cdc42 and PAK are activated by contact disassembly and contribute to the ensuing activation of the SMA promoter. (A) Confluent LLC-PK1 cells were serum-deprived and either incubated in SFM or LCM for the indicated times. Active Rac/Cdc42 was captured by PBD-GST beads. (B) Densitometric analysis of Rac activation is expressed as fold increase over control (mean ± S.E., n = 3). (C) Cells were transfected with PBD-YFP and treated with LCM. Images of the transfected cells (within a confluent monolayer) were taken at the indicated times. (D) Active PAK1 was detected by a phospho-specific antibody. (E) Cells were cotransfected with pSMA-Luc and pRL-TK plasmid along with either empty vector (Control) or with one of the test plasmids, as indicated. Cells were incubated in SFM or LCM, and 24 h later luciferase assays were performed.