Figure 2.

Western blot showing the incorporation levels of recombinant TnT in α- and β-MHC fiber bundles. Reconstituted fiber bundles were solubilized in 2.5% SDS solution and their final concentrations were adjusted to 2 mg/ml. Equal amounts (5 μg) of standardized protein samples were loaded and run on an 8% small SDS-gel for optimal separation of recombinant and endogenous TnT. Proteins were then transferred onto a PVDF membrane and TnT was probed using an anti-TnT primary, followed by an anti-TnT secondary antibody.