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. 2016 Oct 4;7:443. doi: 10.3389/fphys.2016.00443

Figure 3.

Figure 3

Effect of TnTR134W on pCa-tension relationships in α- and β-MHC fiber bundles. Steady-state tensions, normalized to the value at pCa 4.3, were plotted against pCa to construct the pCa-tension relationship. Hill model was fitted to these pCa-tension relationships to estimate pCa50 (myofilament Ca2+ sensitivity) and nH (myofilament cooperativity). TnTR134W-mediated impact on pCa-tension relationship in (A) α-MHC and (B) β-MHC fiber bundles. Traces connecting the data (empty or filled circles) are the Hill model fits. TnTR134W-mediated impact on (C) pCa50 and (D) nH in α- and β-MHC fiber bundles. Statistical differences were analyzed by two-way ANOVA and subsequent post-hoc t-tests using Fishers LSD method. ***P < 0.001 indicate a significant result compared to TnTWT (NS, not significant). The number of fiber bundles measured is as follows: 13 for α-MHC+TnTWT, 12 for α-MHC+TnTR134W, 14 for β-MHC+TnTWT, and 14 for β-MHC+TnTR134W. Data are presented as mean ± SE. Standard error bars are smaller than symbols in some cases.