FIGURE 2.

NF-κBp65 binding activity levels are inversely correlated with intracellular ATP levels in quiescent fibroblasts. ATP concentrations were reduced following treatment of mitochondrial uncouplers (DNP, FCCP) and/or glycolysis inhibitor (2DG) at different concentrations as indicated. NF-κBp65 DNA binding activity was moderately increased following separate treatment with mitochondrial uncoupler (DNP/FCCP) or glycolysis inhibitor (2DG), or low concentrations of combined inhibitors. Marked increase in NF-κBp65 DNA binding activity occurred for combined treatment with mitochondrial uncouplers (DNP/FCCP) and glycolysis inhibitor (2DG) at higher concentrations. Results shown represent means and standard deviations from three independent experiments, and the best fit between ATP concentrations and NF-κBp65 DNA binding activity is an exponential function (NF-κB = 3.52 e^-1.43ATP, R² = 0.86).