FIGURE 2.

Screen for endophyte strain 3A12 mutants that show loss or reduction in anti-fungal activity. (A) Tn5 insertion strains growing on LB-kanamycin agar. (B) Example PDA agar plate containing S. homoeocarpa pathogen showing loss of inhibition zones associated with a candidate mutant compared to wild type 3A12. (C) Summary of candidate 3A12 mutants showing lost or reduced inhibition zones of S. homoeocarpa growth. Asterisks indicate significant difference in mean diameter of the inhibition zone compared to the wild type strain. (D–Q) In planta confirmation of loss of antifungal activity in creeping bentgrass following seed coating with each endophyte strain as indicated. The numbers shown indicate the number of tubes with sick plants out of three tubes.