Fig. 3.

H19 knockdown led to apoptosis in glioblastoma (GBM) cells. a Apoptosis of U87MG and U251 cells with H19 knockdown was measured by Annexin V-FITC/PI double staining assay. TMZ-treated U87MG and U251 cells transfected with non-specific control siRNA and non-treated (NT) were both included as negative controls. Three independent experiments were done; representative flow cytometry data were shown here. b Protein expression level of pro caspase 3, cleaved caspase 3, BCL-2 and BAX was measured in U87MG and U251 cells with H19 knockdown. U87MG and U251 cells transfected with non-specific control siRNA and no siRNA (NT) were both included as negative controls