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. 2016 Mar 16;36(8):1219–1227. doi: 10.1007/s10571-015-0320-5

Fig. 4.

Fig. 4

Cancer stem cell (GSC) markers were downregulated in glioblastoma (GBM) cells with H19 knockdown. a mRNA level of CD133, Nanog, Oct-4 and Sox2 was measured in U87MG and U251 cells with H19 knockdown. U87MG and U251 cells transfected with non-specific control siRNA and no siRNA (NT) were both included as negative controls. Data here represent an average of three independent experiments; error bar indicates standard deviation of the mean; *p < 0.05; # p < 0.005. b Protein level of CD133, Nanog, Oct-4 and Sox2 was measured in U87MG and U251 cells with H19 knockdown. U87MG and U251 cells transfected with non-specific control siRNA and no siRNA (NT) were both included as negative controls. c mRNA level of CD133, Nanog, Oct-4 and Sox2 was measured in U87MG and U251 cells treated with temozolomide (TMZ). TMZ-alone and TMZ + control siRNA groups were both included as negative controls. Data here represent an average of three independent experiments; error bar indicates standard deviation of the mean; *p < 0.05