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. 2016 Jun 17;29(10):1143–1154. doi: 10.1038/modpathol.2016.112

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Copyright © 2016 United States & Canadian Academy of Pathology

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Validation of inter- and intraoperator variability for Tissue Studio analysis of Nuc-pYStat5 in breast cancer by fluorescence immunohistochemistry analysis. (a) Two operators (ARP and HR) followed the same standard operating procedure for Tissue Studio image analysis of levels of Nuc-pYStat5 within 336 breast cancer specimens in tissue microarray format, independently selecting tumor tissue features from 12 of the specimens for region-of-interest training according to established guidelines. Interoperator concordance analysis showed high concordance correlation coefficient of 0.993 (95% confidence interval: 0.991, 0.994). (b) Intraoperator concordance analysis showed high concordance correlation coefficient of 0.996 (95% confidence interval: 0.995, 0.997) on repeated analysis by the same operator (ARP) using Tissue Studio image analysis. Solid line indicates the line of perfect agreement.