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. 2016 Sep 12;5(9):e258. doi: 10.1038/oncsis.2016.59

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Copyright © 2016 The Author(s)

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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ARHGEF5 KD attenuates TGF-β-induced EMT and cell migration in MCF10A cells. (a) ARHGEF5 was knocked down with shRNAs (shGEF5 no. 1 and shGEF5 no. 2) in MCF10A cells. Mock and ARHGEF5 KD cells were treated with or without TGF-β1 for 24 h and the levels of the indicated proteins analyzed by western blotting. (b) Mock and ARHGEF5 KD cells were treated with or without TGF-β1 for 48 h and the cells stained with anti-N-cadherin or anti-E-cadherin. Scale bar: 20 μm. (c) Mock and ARHGEF5 KD cells were treated with or without TGF-β1 for 24 h and subjected to wound-healing assays. Scale bar: 200 μm. (d) The migration rates of the indicated cells are shown. Values represent the mean±s.d. (n=3, ***P<0.001).