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. 2016 Sep 12;5(9):e258. doi: 10.1038/oncsis.2016.59

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Copyright © 2016 The Author(s)

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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ARHGEF5 promotes tumorigenesis of Src-activated fibroblasts. (a) Schematic structures of wild-type ARHGEF5 (ARHGEF5-FULL) and its mutants lacking the DH domain (ΔDH) and Src/PI3K binding domain (Δ583–902). (b) NIH3T3 cells expressing the indicated constructs of ARHGEF5 were treated with or without 4-hydroxy tamoxifen (4-OHT) and ARHGEF5 was immunoprecipitated with an anti-FLAG antibody. Total cell lysates (TCLs) and the bound proteins were analyzed by western blotting. (c) NIH3T3 cells expressing the indicated ARHGEF5 constructs were subcutaneously inoculated into nude mice. Tumors generated 2 weeks after inoculation were excised and photographed (upper). Tumor length (L) and width (W) were measured and tumor volume was evaluated using the mathematical formula V=0.5 × L × W² (lower). Values represent the mean±s.d. (n=4, ***P<0.001).