Table III.
Proton pumping and NADH-oxidase activities of the NuoL mutant complex I reconstituted into UQ- and MK-rich DKO membranes
| Sample | Trial | UQ-rich DKO membranes |
MK-rich DKO membranes |
% (Menaq/Ubiq) | ||||
|---|---|---|---|---|---|---|---|---|
| Initial acidification rate (IAR) | NADH-oxidase activity (NOA) | IAR/NOA | Initial acidification rate (IAR) | NADH-Oxidase activity (NOA) | IAR/NOA | |||
| WT | 1 | 3,474.9 | 3.28 | 1,058.2 | 2,156.9 | 4.28 | 503.5 | 47.6 |
| 2 | 2,682.9 | 5.01 | 535.9 | 1,748.1 | 5.66 | 308.6 | 57.6 | |
| 3 | 4,139.2 | 3.97 | 1,043.2 | 3,313.1 | 5.35 | 619.5 | 59.4 | |
| D178N | 1 | 2,491.4 | 3.06 | 814.7 | 2,541.9 | 2.98 | 854.2 | 104.8 |
| 2 | 2,529.7 | 4.46 | 566.6 | 2,756.0 | 4.71 | 585.2 | 103.3 | |
| 3 | 3,392.0 | 3.10 | 1,095.3 | 3,628.2 | 4.64 | 782.2 | 71.4 | |
| H334Q | 1 | 2,095.5 | 2.94 | 713.9 | 2,309.3 | 4.21 | 549.0 | 76.9 |
| 2 | 1,974.7 | 2.78 | 710.1 | 2,178.0 | 4.17 | 521.8 | 73.5 | |
| 3 | 1,965.4 | 2.86 | 686.1 | 1,901.2 | 4.20 | 452.7 | 66.0 | |
| D400A | 1 | 2,456.7 | 4.19 | 586.3 | 2,621.8 | 5.35 | 490.1 | 83.6 |
| 2 | 2,579.5 | 4.13 | 624.6 | 2,658.6 | 5.68 | 468 | 74.9 | |
| 3 | 2,729.6 | 4.13 | 660.9 | 2,990.7 | 5.29 | 565.3 | 85.5 | |
The values are the average of n = 3–4 measurements.