Table 2. PCR primer specifications.

Primer	Sequence 5′-3′ [bp]	Combinations	T (°C)	Product length (bp)
PRDM9-F1	GGGGTAGGAATCTCAAGAATTG[22]	F3-R1	54	594 (5′ end)
PRDM9-F2	AAGAATTGCAAGGGTCAAGTATG[23]	F1/F2-R3	53	977–1481/963–1467
PRDM9-F3	TGGTGGGAGACAGCTTGC[18]	F3-R3	53	1501–2005
PRDM9-F4	GCCAAGAGGACGAGTAAGTCAATAG[25]	F5-R4	56	1152–1656
PRDM9-F5	CAAAGATCCACCCATGTTC[19]	F4-R2	57	198 (3′ end)
PRDM9-R1	TGACTCATCACTGAGGCCTTG[21]
PRDM9-R2	CCTCATCTCTAGTCATGAAAGTGG[24]
PRDM9-R3	GAAGCACCCTCCAAGCTG[18]
PRDM9-R4	GGTCTCTTTACACTCTTGSAGTC[23]

Given are applied primer combinations, annealing temperatures and product lengths.