Figure 1. Generation of the Ntrk1 knockin mice.

(a) Schematic of the target site at the Ntrk1 locus. In the double-stranded DNA, the PAM sequence is capitalized, and the sgRNA target is underlined. In the donor DNA, the single-strand oligodeoxynucleotide, the replaced nucleotides are capitalized (for KI, green; for creating the underlined TfiI site, red). (b) Sequence chromatograms for the target site of wild-type mice (top), Neuro2A cell line transfected with the CRISPR/Cas expression vector (middle), and heterozygous (F1) KI offspring (bottom). , nucleotide deletion (3 bp); , , nucleotide changes.