Skip to main content
. 2016 Oct 4;6:34827. doi: 10.1038/srep34827

Figure 3. TRIM27 promotes the activation of JNK and p38 pathways and suppresses NF-κB activation.

Figure 3

(a,b) Luciferase assay of of AP-1 (a) and NF-κB (b) activation in U937 cells. Cells were transfected with luciferase siRNA (NC siRNA) or TRIM27 siRNA or TRIM27 siRNA complemented with TRIM27 (ΔRING), and then were infected with WT BCG or BCG (ΔPtpA) or the BCG (ΔPtpA) complemented with PtpA D126A (ΔPtpA+D126A) at a MOI of 10 for 24 h. (c) Immunoblot analysis of phosphorylated IκBα, JNK, p38, Erk and GAPDH (loading control) in U937 cells transfected with luciferase siRNA (NC siRNA) or TRIM27 siRNA and infected with BCG at a MOI of 10 for 0–48 h. Non-infected cells served as a control group. Data are shown as the means ± s.e.m. *P < 0.05 and **P < 0.01.