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. 2016 Oct 4;6:34713. doi: 10.1038/srep34713

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Copyright © 2016, The Author(s)

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Cells were co-cultured with different material extracts supplemented with serum-free α-MEM. Normal culture medium containing α-MEM supplemented with 10% FBS was used as blank control medium (Control/N). Cells cultured with osteogenic differentiation medium were used as positive controls (Control/M). ALP staining after 9 days of cultivation was performed using an ALP Staining Kit (A). ALP activity was evaluated at 3 days, 5 days, 7 days, and 9 days using an ALP Kit (B). The results are expressed as means ± standard deviation and *P < 0.05 represents a significant difference compared with the control/N group.