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. 2016 Oct 4;6:34695. doi: 10.1038/srep34695

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) The relative bioluminescence intensity (RBI) of V. harveyi cells in aggregate with different sizes. (b) The relative AI-2 induction of different aggregate sizes. AI-2 induction was quantified using a biological method employing V. harveyi BB170 as a reporter strain, and the supernatant from BB152 was used as a positive control. (c) The expression levels of QS-related genes in aggregates with different sizes. The RNA polymerase A subunit gene (rpoA) was used as a housekeeping gene, and the results were normalized to the SA group. Genes in green color indicated down-regulated expression and genes in red color indicated up-regulated expression. Error bars represent the standard deviation of three replicates. Significant differences between groups were analyzed by t-test. Single asterisk indicates significant difference (p < 0.05) and double asterisk indicates significant difference (p < 0.01).