Figure 7. Modification of cytosine 34 modulates codon recognition by mt‐tRNA^{M et} in vitro .

1. MTIF2‐dependent reading of initiation codons AUG, AUA or AUU in the P site of the ribosome by unmodified (unmod.) or C34‐modified [¹⁴C]Met‐tRNA^Met. Binding was determined by nitrocellulose filtration, and [¹⁴C]Met‐tRNA^Met retrieved on the membrane was quantified by scintillation counting. Binding in the absence of ribosomes (no 70S) or mRNA (no mRNA) served as controls. Data from three independent experiments are presented with error bars indicating ± SEM. The statistical significance of the results was analysed by t‐test and is indicated by the asterisks in the graph (*P < 0.05).
2. TUFM‐dependent recognition of A site codons during elongation. Data from three independent experiments are presented with error bars indicating ± SEM and statistical analysis as in (A) (*P < 0.05, **P < 0.01).