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. 2016 Aug 6;35(19):2068–2086. doi: 10.15252/embj.201593083

Figure 1. Mutants of B1‐type cyclins are hypersensitive to cisplatin.

Figure 1

  • A
    cycb1;1, cycb1;2, cycb1;3, cycb1;4, cycb1;1/1;2, cycb1;1/1;3, cycb1;1/1;4, cycb1;2/1;4, cycb1;3/1;4, and the wild type (from left to right) on control plates without genotoxic agent 10 days after germination.
  • B
    The wild type, single, and double mutants of cycb1 were grown on control plates without genotoxic agent. Root lengths were measured 10 days after germination.
  • C
    The wild type, cycb1;1, cycb1;2, cycb1;3, cycb1;4, cycb1;1/1;2, cycb1;1/1;3, cycb1;1/1;4, cycb1;2/1;4, cycb1;3/1;4 (from left to right) on plates containing 1 mM hydroxyurea (HU) 10 days after germination. The rightmost plant is the wee1 mutant that shows high sensitivity to HU.
  • D
    The wild type, single, and double mutants of cycb1 were grown on plates supplemented with 1 mM HU. Root lengths were measured 10 days after germination.
  • E
    The wild type, cycb1;1, cycb1;2, cycb1;3, cycb1;4, cycb1;1/1;2, cycb1;1/1;3, cycb1;1/1;4, cycb1;2/1;4, cycb1;3/1;4 (from left to right) on plates containing 0.6 μg/ml bleomycin (BLM) 10 days after germination. The rightmost plant is the ku70 mutant that shows high sensitivity to BLM.
  • F
    The wild type, single, and double mutants of cycb1 were grown on plates supplemented with 0.6 μg/ml BLM. Root lengths were measured 10 days after germination.
  • G
    The wild type, cycb1;1, cycb1;2, cycb1;3, cycb1;4, cycb1;1/1;2, cycb1;1/1;3, cycb1;1/1;4, cycb1;2/1;4, cycb1;3/1;4 (from left to right) on plates containing 15 μM cisplatin 6 days after germination, that is, 3 days after transfer from control plates.
  • H–J
    cycb1 mutants were germinated on control plates and were transferred to new control plates (H) or plates supplemented with 15 μM (I) or 30 μM (J) cisplatin 3 days after germination. Root lengths were measured 3 days after transfer and the net root growth of 3 days is shown in the graphs.
  • K
    The wild type, cycb1;1, cycb1;2, cycb1;3, cycb1;4, cycb1;1/1;2, cycb1;1/1;3, cycb1;1/1;4, cycb1;2/1;4, cycb1;3/1;4 (from left to right) on plates containing 30 μM cisplatin 6 days after germination, that is, 3 days after transfer from control plates.
  • L–N
    cycb1 double mutants germinated on control plates and were transferred to new control plates (L) or plates supplemented with 15 μM (M) or 30 μM (N) cisplatin 3 days after germination. Root lengths were measured 3 days after transfer and the net root growth of 3 days is shown in the graphs.
Data information: One or two asterisks indicate significant differences within a 5 and 1% confidence interval, respectively (Student's t‐test). Scale bars: 1 cm. Three biological replicates, each containing at least 15 plants, were analyzed. The mean of the root length of each individual experiment was determined and again averaged for the three biological replicates. Graphs represent mean ± SD.