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. 2016 Aug 6;35(19):2068–2086. doi: 10.15252/embj.201593083

Figure 3. Homologous recombination frequencies are strongly reduced in cycb1 mutants.

Figure 3

  • A
    Schematic drawing of homologous recombination assay. Restoring the functional GUS gene from two disrupted parts (GU' and US') is restricted to an intermolecular homologous recombination event. Homologous events occur only when a sister chromatid or homolog is available as a template, that is, in G2 phase of the cell cycle.
  • B
    Wild‐type plants show blue spots on the leaves after 3 days of incubation on 30 μM cisplatin. Arrows indicate representative blue sectors.
  • C
    cycb1;1 plants show blue spots on the leaves after 3 days of incubation on 30 μM cisplatin. Arrows indicate representative blue sectors.
  • D–F
    Graphs show numbers of blue sectors per plant grown without drug treatment (D) or after incubation on 15 μM (E) or 30 μM (F) cisplatin for 3 days. One or two asterisks indicate significant differences within a 5 and 1% confidence interval, respectively (Student's t‐test). Three biological replicates, each containing at least 15 plants, were analyzed. The mean of the root length of each individual experiment was determined and again averaged for the three biological replicates. Graphs represent mean ± SD.